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Form of workWe are seeking an outstanding Staff Scientist, BioProcess Development to join the New Product Development team to support the development of custom biological reagents. This is a unique cross-disciplinary position that will work closely with a variety of groups including Protein Engineering, Molecular Biology, Chemistry, QC, and Manufacturing, to produce protein and enzyme reagents for the rapid expansion of 10x product lines. Candidates must have an expert working knowledge of protein expression, purification, and formulation and be able to apply that knowledge creatively and independently in a fast-paced work environment. The successful applicant will have exceptional attention to detail and the ability to meticulously execute and analyze highly complex experiments.
Individuals applying for this position must be self-starters, strategic thinkers, action driven, flexible in changing direction and methods, thrive in a fast-paced environment, excel at managing multiple priorities, succeed in communicating with all levels within the organization and provide immediate contribution.
What you will be doing:
- Provide hands on leadership in bench scale fermentation (1-20L), harvesting, purification, and quality control of recombinant proteins for molecular biological workflows.
- Develop and optimize protein purification processes in a pilot production environment.
- Use analytical methods such as HPLC, SDS-PAGE, enzyme activity, nuclease activity, and purity at various stages of the development process.
- Subject matter expert and lead technical resource for team regarding protein reagent development projects and process transfer to manufacturing.
- Day to day management of protein production lab
- Help in leading infrastructure requirements to build out internal manufacturing capabilities.
- Process and knowledge transfer to production and QC staff through documentation and hands on training
Minimum Requirements:
- BS with 15+ years, MS with 12+ years or PhD with 8+ years industry experience in expressing, purifying, and analyzing enzymes relevant to molecular biology workflows.
- Strong technical knowledge and direct hands-on experience with pilot and production scale downstream processing operations such as chromatography, tangential-flow filtration, centrifugation, formulation, etc.
- A solid enzymology background is essential.
- Understanding of engineering issues related to pilot scale processes and ability to apply both practical and analytical skills to implement relevant scaling models.
- Sound understanding of protein biochemistry, recombinant protein expression in microbial hosts, and molecular biology of life science enzymes and formulations.
- Experience with DOE methodology for improving titer, protein formulation and protein stability.
- Solid protein analytical experience such as SDS PAGE, Western Blot, FPLC, HPLC, MS, etc
- Hands-on experience with AKTA chromatography systems, including the design and troubleshooting of protein purification workflows using and operating Unicorn software packages.
- Demonstrated record of successful protein production transfer from late research stages into a manufacturing scale environment.
- Interact with suppliers, operations, logistics, QC, EHS, and R&D
- Proven ability to write Batch Records, BOMs and train others to successfully perform the work.
- Exceptionally strong collaboration and communication skills
Preferred skills/experience:
- Experience in an ISO manufacturing setting and good documentation practices.
- Previous experience with custom and/or novel enzyme formulations.
- Experience working with 10L-20L scale fermentation units.
- Familiar with methods for chromatography unit operations, from screening and characterization to optimization and scale-up
- Experience operating liquid handling robots and other laboratory automation tools. Experience programming these instruments would be highly beneficial.
- Experience working with microfluidic devices.
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