Senior Staff Research Associate

About Us: Our lab is dedicated to advancing the understanding of epithelial biology in lung repair and regeneration, particularly focusing on the impact of aging and fibrosis. We are actively involved in developing novel drugs and molecular tools to modulate the repair process and prevent fibrosis. See recent publications: Chapman et al NEJM, 2020; Kathiriya et al CSC, 2020 and NCB, 2022; LeSaux et al AJRCMB 2024). With ongoing projects investigating alveolar repair in both normal and diseased human lungs, we offer a unique opportunity to delve into groundbreaking research. As a Research Assistant, you will play a pivotal role in our research endeavors. You will gain hands-on experience in various techniques including culturing lung slices, isolating primary cells from human lungs, and analyzing samples using flow cytometry and immunofluorescence. Additionally, you will manage mouse strains, conduct genotyping, and assist in testing transgenic mouse lines. This position offers an exciting opportunity to develop valuable skills in cell biology, setting a solid foundation for future graduate studies or corporate employment.

Position Overview:

Qualifications:

• Bachelor's degree or equivalent with training in cell and molecular biology
• Prior lab experience is required, experience in the field of lung biology is a plus but not required.
• Strong organizational skills and attention to detail
• Ability to work effectively within a team environment
• Eagerness to learn and contribute to cutting-edge research

Responsibilities Include:

• Isolation and culture of pulmonary cells from human lungs under varying conditions, including co-cultured systems such as organoids
• Preparation of precision cut lung slices and culture under varying conditions
• Isolation and preservation of mouse lungs for biochemical and histological assays
• Isolation of RNA, DNA, and protein from various cells and tissues
• Immunofluorescence and X-gal staining of primary cells, cell lines, and lung sections. Imaging slides on a Zeiss microscope.
• Genomic DNA isolation and PCR analysis for human and mouse samples

Applicants may be in process of obtaining their required degrees and/or years of experience in the relevant specialization at the time of application, however, these qualifications must be obtained by appointment start date.

Salary commensurate with the UCSF salary scale plus housing supplements.

The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.

Job Type: Full-time

Benefits:

• Commuter assistance
• Dental insurance
• Health insurance
• Paid time off
• Retirement plan
• Vision insurance

Schedule:

• Monday to Friday

Ability to Relocate:

• San Francisco, CA 94143: Relocate before starting work (Required)

Work Location: In person