- Execute various cellular and molecular techniques to validate genetic or small molecule perturbations of targets and downstream pathways such as: western blot, qPCR, ELISA, MSD, immunoprecipitation, gene expression, luminescence-based assays, imaging platforms and flow cytometry
- Carry out experiments to test the functional consequences of genetic (CRISPR-based gene editing technologies and RNA interference) and chemical perturbations in cell-based model systems
- Develop cellular tools for advancing drug discovery projects including generation and maintenance of engineered cell lines in a wide array of established cancer types
- Serve as a technical resource within the group for engineering and generating stable cell lines, genome-wide gene editing experiments, phenotypic and biomarker assay development, or other technologies
- Design and conduct well controlled high-quality scientific experiments to test biological hypotheses
- Assist with the evaluation of new approaches and technologies within the group
- Maintain electronic lab notebook in compliance with company policies
- Stay current with relevant technologies including those relating to molecular biology, cell biology, functional genomics and biochemistry
- Work closely as a collaborative team member within Cancer Cell Biology and Oncology project teams
- Bachelor’s degree in cell and molecular biology or related field with at least 6+ years laboratory experience in an industrial or academic setting or master’s degree with at least 3+ years laboratory experience, experience in oncology research is a plus
- Expertise with an array of molecular biology methods including cloning, vector construction, PCR, DNA and RNA isolation, qPCR, and library construction
- Expertise with a variety of cell biology techniques including cell culture, engineering and generation of stable cell lines, lentiviral production, gene expression analysis, protein expression analysis, and cell-based assays, phenotypic assays, biomarker assays, luminescence-based assays, reporter assays, imaging assays, and flow cytometric assays
- Experience in CRISPR-based approaches or RNA interference technologies
- Expertise in transcription factors, epigenetics or E3 ubiquitin ligase fields is a plus
- Track record of successful contribution to research projects, preferably utilizing gene editing or screening methodologies
- Ability to effectively interact and collaborate with other scientists, both within the group and in other groups; excellent communication and interpersonal skills
The annual base salary for this position ranges from $74,900.00 to $124,800.00. In addition, this position is eligible for participation in Pfizer’s Global Performance Plan with a bonus target of 7.5% of the base salary. We offer comprehensive and generous benefits and programs to help our colleagues lead healthy lives and to support each of life’s moments. Benefits offered include a 401(k) plan with Pfizer Matching Contributions and an additional Pfizer Retirement Savings Contribution, paid vacation, holiday and personal days, paid caregiver/parental and medical leave, and health benefits to include medical, prescription drug, dental and vision coverage. Learn more at Pfizer Candidate Site – U.S. Benefits | (uscandidates.mypfizerbenefits.com). Pfizer compensation structures and benefit packages are aligned based on the location of hire. The United States salary range provided does not apply to Tampa, FL or any location outside of the United States.