Address
Form of work
GENERAL SUMMARY/ OVERVIEW STATEMENT:
The Cotman laboratory seeks a Research Technician to work with investigators and other laboratory staff to perform laboratory-based research aimed at delineating the molecular basis of Neuronal Ceroid Lipofsucinosis (NCL, Batten disease), which is an inherited neurodegenerative disorder affecting children. The research specifically involves utilizing genetic mouse and cell culture models of NCL, including iPS, neuronal and microglial cultures. The goal of our research is to apply genetics, cell biology, and biochemical studies to characterize the genetic basis of NCL to translate these discoveries to improvements in clinical care of those affected by NCL. We are seeking a highly motivated individual to join our team of talented and diverse researchers in a collaborative research environment.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Independently conduct cell culture and mouse-based experiments
Maintain breeding colony of mice and perform general health inspection of mice and maintain findings in records
Perform standard molecular biological techniques, including PCR, DNA extraction, genotyping, protein lysate preparation, Western blotting, and qPCR.
Perform tissue processing and histological experiments with mouse tissues
Analyze results of experiments and record details of experiments and results using electronic lab notebook system.
Analyze histological studies by light, epifluorescence and confocal microscopy.
Contribute to troubleshooting and developing new laboratory processes and technologies.
Interface with MGH animal facility staff on a regular basis.
Attend and participate in lab meetings, including preparing reports and presenting progress.
Assist in preparation of manuscripts, poster presentations, and project reports.
Perform ordering and regularly inventory laboratory supplies.
SKILLS/ABILITIES/COMPETENCIES REQUIRED:
Proficiency in molecular biology techniques and previous experience with protein isolation, Western blotting, PCR, genotyping, and/or DNA extraction a plus.
Ability to handle a variety of tasks amid shifting priorities.
Strong analytical skills with a high degree of initiative.
Creative and highly motivated individual with strong organizational and management skills.
Excellent written and verbal communication skills.
Ability to work independently
EDUCATION:
B.Sc. in Biology, Genetics, Neuroscience or related discipline
EXPERIENCE:
Some experience (even less than a year) with molecular biology techniques including cell culture preferred.
WORKING CONDITIONS:
Research will be conducted in the Cotman laboratory, located on the 5th floor of the Simches Research building, and in the MGH animal facility.
