Research Technician I

GENERAL SUMMARY/ OVERVIEW STATEMENT: 
We are seeking a motivated, enthusiastic and experienced Research Technician to join the Rosas lab in the Department of Neurology at the Massachusetts General Hospital MIND Center, Harvard Medical School. Under the mentorship of Dr. H. Diana Rosas, MD, the lab uses and develops techniques and approaches to identify blood and CSF biomarkers from human samples for the determination of key factors that determine onset and/or progression in Huntington's disease and in Alzheimer's disease in Down syndrome. Insights from these studies are then used to further investigate salient and key pathological mechanisms that underlie phenotypic variation and neurodegeneration.

Under the direction of the Principal Investigator and post-doctoral fellows, the Research Technician will perform laboratory tests on longitudinally collected human samples which include DNA and RNA purification, isolation and characterization of small molecules by HPLC, preparation of libraries for RNA seq, RT- PCR, gel electrophoresis, Western blots and ELISA. Prospective applicants will receive the necessary training to successfully accomplish these analyses. Additional responsibilities include ordering necessary reagents to conduct experiments, and participation in shared upkeep of the lab.  Experience in RNA seq and/or reverse phase analytical techniques is preferred but not required. The individual must be attentive to detail, be able to work both independently and as part of a group, be able to communicate effectively and be responsible and diligent.  This is an ideal position for someone wishing to pursue and academic advanced degree or to assume greater autonomy and responsibility within the lab.

PRINCIPAL DUTIES AND RESPONSIBILITIES: 
Specific Responsibilities include but are not limited to:

• Purification and quality control of high-quality RNA from blood and other samples following standard protocols.
• Characterization of small molecules by HPLC and other analytical techniques.
• Performs standard laboratory procedures such as DNA extraction, library preparation, RT PCR and droplet digital PCR.
• Perform routine molecular, and cellular based assays such as Western blots, plate-based assays, and protein determinations.
• Organizes and accurately maintains written records of experimental procedures and data collection. Data may be represented as graphs, charts, tables, illustrations, slides, and narrative reports
• Assist in preparation of manuscripts resulting from data generated in research projects
• May share responsibility for ordering common laboratory reagents and general maintenance of the laboratory
• Participates in upkeep of lab, performs basic maintenance on laboratory equipment and reports equipment requiring advanced repair
• Performing other job-related duties as assigned.

SKILLS & COMPETENCIES REQUIRED: 

• Demonstrated knowledge of general molecular biology techniques like RNA /DNA extractions, HPLC, RT-PCR and western blot.
• Must be able to multi-task with great efficiency
• Excellent organizational and communication skills (articulate with good writing and phone skills)
• Must have a professional demeanor and a strong commitment to confidentiality
• Attention to detail
• Excellent time management skills
• Knowledge of standard laboratory techniques
• Computer skills: MS Word and Excel
• A background in statistics and/or data analytical techniques a plus (including AI, or other advanced computational methods).

EDUCATION:

Bachelor's Degree required.

EXPERIENCE:

One to two years of previous experience in molecular biology strongly preferred.

WORKING CONDITIONS:

The employee is required to work with hazardous materials such as chemicals and biological fluids.