Scientist I, Genome Editing

Company Summary:
Located in San Diego, CA, Shoreline Biosciences, Inc. is focused on changing the lives of patients and families living with cancer by developing safe, highly effective, and accessible allogeneic NK and macrophage cell therapies. Shoreline was recognized by the California Life Sciences as the Pantheon 2021 award winner for the Strategic Partnership of the Year Award. Join our winning team!
Position Summary:
Shoreline Biosciences is seeking a skilled and highly motivated candidate to join our growing R&D team at the Scientist I level. This position will play a pivotal role in the development of life-saving, cutting-edge induced pluripotent stem cell (iPSC)-derived Natural Killer Cell and Macrophage therapies. The primary role of the candidate will be to perform genomic characterization of engineered iPSCs and immune cells via Next Generation Sequencing (NGS). The ideal candidate will be motivated to learn new techniques and analysis methods and support the Genome Editing team in multiple activities such as CRISPR engineering, NGS data analysis, iPSC cell characterization, and to contribute to process improvements and technology development. This will be a full-time position reporting directly to the Senior Scientist. The anticipated salary range for this position is $75,000 to $138,000. The final salary offered to a successful candidate depends on the candidate's experience, qualifications, geographic location, and other job-related factors. In addition to base salary, the hired candidate is eligible to receive an annual bonus and will receive an equity grant, at the time of hire, in the form of the option to purchase stock in the future for a specified price.
Primary Responsibilities:

• Support the Genome Editing team in the genetic characterization of genome-edited iPSC via standard molecular biology techniques and Next Generation Sequencing.
• Perform NGS library preparation and sequencing (Whole Exome Sequencing, RNA-Seq, rhAMP-Seq).
• Keep accurate records of samples and reagents.
• Manage daily operations of sequencing activities.
• Depending on expertise, perform downstream bioinformatics analysis of in-house generated as well as external datasets.
• Additional support in human iPSC cell culture in R&D and/or GMP settings and CRISPR engineering may be required depending on expertise.

Qualifications:
Education & Experience

• MS in Molecular Biology, Genetics, Biomedical Engineering, Bioinformatics or a related science with 5+ years relevant experience; or PhD in the same disciplines.
• Demonstrated experience with Next Generation Sequencing library preparation methods.
• Experience with molecular biology techniques (nucleic acids extraction and quantification, PCR, qPCR).
• Experience in bioinformatics and NGS data analysis processes such as alignment and variant calling.
• Experience with liquid handling systems and automation.
• Experience in iPSC, ESC, or other stem cell culture.
• Experience in CRISPR engineering.

Skills & Knowledge

• Coding skills in R or Python, bash and standard bioinformatics tools.
• Knowledge of standard laboratory and safety procedures.
• Aptitude for technology and instrumentation.
• Strong organizational skills, high level of data integrity and attention to details.
• Comfortable in a fast-paced work environment and able to adjust workload based upon changing priorities.
• Excellent interpersonal, verbal, and written communication skills.

Physical Demands:
The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

• While performing the duties of this job, the employee is regularly required to talk or hear. The employee is frequently required to sit.
• The employee is required to stand; walk; use hands to finger, handle, or feel and reach with hands and arms. Transition from standing and sitting often.
• Specific vision for this job includes close vision, distance vision, color vision, peripheral vision, depth perception and ability to adjust focus.
• This position requires the ability to occasionally lift and/or move up to 10 pounds.

Work Environment:

• Will require working with cells and cell lines of human and/or animal origin.
• Will require working with hazardous materials.
• Work in and around common laboratory equipment.
• The regular requirements of an office environment (computers, computer screens, workstations, etc.) apply when not working in or around a laboratory environment.
• 100% on-site work at corporate headquarters in San Diego, CA.
• Evening and weekend work as necessary.

Disclaimer:
The above statements are intended to describe the general nature and level of work performed by employees assigned to this classification. They are not intended to be construed as an exhaustive list of all responsibilities, duties and skills required of personnel so classified.
Visit our website to find out more about Shoreline Biosciences: https://www.shorelinebio.com/
EEO Employer: Shoreline Biosciences, Inc., is an equal opportunity employer; all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, veteran status, or any other protected classification.