Cell Therapy Associate Scientist

Janssen Research & Development, L.L.C., a division of Johnson & Johnson's Family of Companies is recruiting for a Cell Therapy Associate Scientistpositionlocated in Spring House, PA.

At the Janssen Pharmaceutical Companies of Johnson & Johnson, we are working to create a world without disease. Transforming lives by finding new and better ways to prevent, intercept, treat and cure disease inspires us. We bring together the best minds and pursue the most promising science. We are Janssen. We collaborate with the world for the health of everyone in it. Learn more at www.janssen.com and follow us @JanssenGlobal. Janssen Research & Development, LLC is part of the Janssen Pharmaceutical Companies.

We seek a motivated and high performing individual to join the Cell Therapy group within Therapeutics Discovery (TD) to support the development of a universal, off-the-shelf iPSC Cell Therapy platform. In this role you will culture, characterize, and differentiate gene-edited iPSCs to help develop optimal genetic engineering approaches. The successful candidate will have a background in Cell & Molecular Biology, Bioengineering, or Immunology and experience culturing iPSCs. Success in this position will require both the ability to independently execute lab responsibilities and the ability to effectively collaborate within a cross-functional team. This role is an exciting opportunity to join a diverse and ambitious discovery team and to work with state-of-art technology to create life-changing therapies for patients.

Key Responsibilities:

• Culture, expand, and bank iPSCs
• Perform single-cell cloning of gene-edited iPSCs and subsequent flow cytometric analysis and molecular characterization
• Differentiate gene-edited iPSCs into hematopoietic stem and progenitor cells (HSPCs)
• Develop and optimize high-throughput screening methods for iPSC differentiation
• Analyze data, prepare reports, and effectively communicate results
• Maintain laboratory records and ensure compliance with laboratory protocols and safety procedures
• Work collaboratively with other team members to achieve project goals

• Bachelor's Degree in Cell & Molecular Biology, Bioengineering, Immunology, or related field is required

Required:

• Experience with iPSC or stem cell culture and differentiation protocols is required
• Background in molecular biology techniques, including PCR, qPCR, and DNA sequencing is required
• Ability to independently perform lab-based responsibilities with limited guidance is required
• Ability to collaborate with others to plan experiments and coordinate frequent hand-offs of cell lines and data packages to members of other teams is required
• Self-motivated with excellent organizational and time management skills is required

• A minimum 2 years of stem cell experience is preferred
• Experience with flow cytometry is preferred
• Experience with gene editing techniques such as CRISPR/Cas systems is preferred
• Experience with automation and high-throughput screening is preferred
• Prior immunology experience, including T cell biology and chimeric antigen receptors is preferred

Johnson & Johnson is an Affirmative Action and Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, or protected veteran status and will not be discriminated against on the basis of disability.