Biological Scientist

Job Family:
Scientist (Digital)
Travel Required:
None
Clearance Required:
Ability to Obtain Public Trust
What You Will Do:
We are currently searching for a Biological Scientist. The individual will independently provide support services to satisfy the overall operational objectives of the National Institute on Aging - Center for Alzheimer's and Related Dementias (CARD). The primary objective is to provide services and deliverables through performance of support services. This is a full-time opportunity located in Bethesda, MD.

• Independently design, troubleshoot, and carry out various molecular cloning experiments related to CRISPR interference tool development and iPSC differentiation.
• Use SnapGene software to design and plan novel cloning projects.
• Work with staff on performing large-scale iPSC cell culture experiments.
• Work with staff on performing various experiments and procedures related to differentiation and phenotyping of induced pluripotent stem cell lines.
• Work independently in designing, developing, and performing new methods.
• Work with staff to characterize genetic pathways for Alzheimer's disease and related dementia (ADRD) using human cell-based models.
• Evaluate the stability of iPSC and iPSC-derived cells across multiple engineered and non-engineered lines.
• Work with staff to apply published protocols for cell differentiation to IPSC lines, microscopy, and molecular analysis.
• Work with staff to provide insight into the effects of ADRD-associated mutations on molecular readouts in cell culture.
• Support other research projects.
• Collect, Analyze, interpret, and organize data and present results in team and departmental meetings.
• Keep detailed, organized, and accurate digital and physical experimental records.
• Update and maintain laboratory notebooks and databases.
• Attend meetings and present results and findings for staff discussion.
• Transform bacteria with agar and liquid culture.
• Perform plasmid miniprep, maxiprep, giga prep using column-based extraction kits.
• Prepare plasmid samples for sequencing by external companies.
• Perform genomic DNA extraction using column-based extraction kits.
• Prepare custom genomic DNA libraries for next-generation sequencing.
• Collaborate with other team members to carry out large-scale cell culture protocols using manual and high throughput robotic systems.
• Transduce iPSCs with lentivirus and transfect iPSCs with plasmids as part of routine cell culture experiments.
• Perform standard immunological techniques, including western blotting and immunostaining.
• Perform flow cytometry on iPSCs.
• Provide scientific and technical expertise to all visiting fellows, post-docs, and technicians internal and external.
• Mentor junior level scientists by supporting other research projects.
• Train staff on the proper usage of laboratory instruments and equipment.
• Calibrate, maintain, and troubleshoot instruments and equipment in the laboratory.
• Maintain and perform staff training on scientific instrumentation, cell culture microscopies, microanalytical balances,
• centrifuges, automated platforms.
• Operate, troubleshoot, and train others to use laboratory equipment and software.
• Provide assistance to laboratory staff in designing experiments, using instrumentation, data acquisition and analysis, and preparing figures for publication.
• Review scientific equipment and resources including reagents, stocks of frozen cells, mammalian samples including acquisition, documentation, and storage.
• Mentor junior level scientists by training them on the proper use of equipment.
• Develop new differentiation protocols for iPSC lines to differentiate into disease-relevant cell types like neurons, astrocytes, and microglia.
• Develop image analysis routines to analyze the effects of disease-associated mutations on cellular phenotypes.
• Willing to learn how to operate, and implement novel methods on liquid handling platforms (Hamilton, Biotek, etc.)
• Willing to learn and maintain in-house flow cytometry machine.

What You Will Need:

• Master's degree in Biology, Cellular Biology, Molecular Biology, Neuroscience, Genetics, Biochemistry, or related discipline.
• At least four (4) years of experience in research or laboratory setting.
• Hands-on experience with molecular cloning design, implementation, and troubleshooting.
• Hands-on experience with cell culture in particular working with iPS cells.
• Excellent analytical, organizational and time management skills.
• MS Office
• SnapGene

What Would Be Nice To Have:

• PhD preferred.
• Knowledge of proper laboratory disposal methods and procedures

#SOAR
#LI-FG1
What We Offer:
Guidehouse offers a comprehensive, total rewards package that includes competitive compensation and a flexible benefits package that reflects our commitment to creating a diverse and supportive workplace.
Benefits include:

• Medical, Rx, Dental & Vision Insurance
• Personal and Family Sick Time & Company Paid Holidays
• Parental Leave
• 401(k) Retirement Plan
• Group Term Life and Travel Assistance
• Voluntary Life and AD&D Insurance
• Health Savings Account, Health Care & Dependent Care Flexible Spending Accounts
• Transit and Parking Commuter Benefits
• Short-Term & Long-Term Disability
• Tuition Reimbursement, Personal Development, Certifications & Learning Opportunities
• Employee Referral Program
• Corporate Sponsored Events & Community Outreach
• Care.com annual membership
• Employee Assistance Program
• Supplemental Benefits via Corestream (Critical Care, Hospital Indemnity, Accident Insurance, Legal Assistance and ID theft protection, etc.)
• Position may be eligible for a discretionary variable incentive bonus

About Guidehouse
Guidehouse is an Equal Employment Opportunity / Affirmative Action employer. All qualified applicants will receive consideration for employment without regard to race, color, national origin, ancestry, citizenship status, military status, protected veteran status, religion, creed, physical or mental disability, medical condition, marital status, sex, sexual orientation, gender, gender identity or expression, age, genetic information, or any other basis protected by law, ordinance, or regulation.
Guidehouse will consider for employment qualified applicants with criminal histories in a manner consistent with the requirements of applicable law or ordinance including the Fair Chance Ordinance of Los Angeles and San Francisco.
If you have visited our website for information about employment opportunities, or to apply for a position, and you require an accommodation, please contact Guidehouse Recruiting at 1-571-633-1711 or via email at RecruitingAccommodation@guidehouse.com. All information you provide will be kept confidential and will be used only to the extent required to provide needed reasonable accommodation.
Guidehouse does not accept unsolicited resumes through or from search firms or staffing agencies. All unsolicited resumes will be considered the property of Guidehouse and Guidehouse will not be obligated to pay a placement fee.