Research Associate

Great opportunity to get your foot in the door with one of the top Biotech companies in the Boston,MA area!

Description:

• Operate in-house processes for circular RNA (circRNA) and mRNA production, including running HPLC or FPLC, collecting and pooling chromatogram peaks, and reporting in LIMS.
• Perform circRNA and mRNA purifications to support chromatography process development and the supply of 1 mg of material to support in-vivo mouse studies and aid in the development and supply of the 1 gram scale.
• Analyze circRNA and mRNA fraction intermediates and final purified material analytically using agarose and PAGE gels, capillary gel electrophoresis (CGE), ELISAs, HPLC and other methods.
• Take ownership in troubleshooting and maintenance equipment•
• Develop processes for verification and quality control and participate in the implementation of quality controls to our assays to ensure low CV and high reproducibility

Skills:

cell culture, mammalian cell culture, RNA, circular rna, HPLC, FPLC, Elisa, gel electrophoresis, Capillary gel electrophoresis, IVT, biochemistry, protein purification, PCR, qPCR, liquid handlers, hamilton

Additional Skills & Qualifications:

• AS or BS in biochemistry, biology, analytical chemistry, chemistry, chemical engineering, or equivalent degree + 2 years of related industry experience.
• Lab experience in any of the following areas: chromatography, purification, enzymatics, analytics and/or general biotech laboratory work.
• AS or BS in biochemistry, biology, analytical chemistry, chemistry, chemical engineering, or equivalent degree + 2 years of related industry experience.
• Lab experience in any of the following areas: chromatography, purification, enzymatics, analytics and/or general biotech laboratory work.
• RNA biochemistry experience
• Experience performing quantitative analyses using any of the techniques: CGE, Agarose Gel, PAGE, ELISA, qPCR/RT-PCR, HPLC / UPLC, SLS, DLS, Tm, SPR, BLI, CD
• automated and/or high throughput liquid handlers (Hamilton, LC autosamplers, automated workcells, etc

Work Environment

M-F 9:00am-5:00pm

Experience Level:

Intermediate Level

Job Type: Full-time

Pay: $30.00 - $45.00 per hour

Expected hours: 40 per week

Benefits:

• 401(k)
• Dental insurance
• Health insurance
• Life insurance
• Paid time off
• Vision insurance

Schedule:

• 8 hour shift

Ability to Relocate:

• Boston, MA: Relocate before starting work (Required)

Work Location: In person