Protein Engineer

Job Title: Protein Engineer
Job Function:
Our client currently has a process to enrich and purify circular RNA at scale and are working to improve it to produce a step-change in production output. The Protein Engineer will operate both existing and future RNA analytics and purification processes as they evolve.
Job Type: Contract
Location: On-Site
Primary Responsibilities:

• Operate in-house processes for circular RNA (circRNA) and mRNA production, including running HPLC or FPLC, collecting and pooling chromatogram peaks, and reporting in LIMS.
• Perform circRNA and mRNA purifications to support chromatography process development and the supply of 1 mg of material to support in-vivo mouse studies and aid in the development and supply of the 1-gram scale.
• Analyze circRNA and mRNA fraction intermediates and final purified material analytically using agarose and PAGE gels, capillary gel electrophoresis (CGE), ELISAs, HPLC and other methods.
• Take ownership of troubleshooting and maintenance equipment.
• Use Client's Software and Automation infrastructure to execute production process and collaborate with software engineers to understand and provide feedback on how to improve upon Client's LIMS and data capture pipeline.
• Work with other team members to drive standardization, quality processes and documentation towards improving our analytical infrastructure.
• Develop processes for verification and quality control and participate in the implementation of quality controls to our assays to ensure low CV and high reproducibility.

Basic Qualifications:

• AS or BS in biochemistry, biology, analytical chemistry, chemistry, chemical engineering, or equivalent degree + 2 years of related industry experience.

• Lab experience in any of the following areas: chromatography, purification, enzymatic, analytics and/or general biotech laboratory work.
• Basic knowledge of rudimentary analytical statistics (standard deviations, coefficients of variance, regression analysis, etc.)

Preferred capabilities and experience:

• RNA biochemistry experience
• Experience performing quantitative analyses using any of the techniques: CGE, Agarose Gel, PAGE, ELISA, qPCR/RT-PCR, HPLC / UPLC, SLS, DLS, Tm, SPR, BLI, CD
• Direct experience analyzing data and reporting on analytical results.
• Prior experience working with automated and/or high throughput liquid handlers (Hamilton, LC autosamplers, automated work cells, etc.
• Demonstrated experience implementing quality controls in wet lab processes.
• Experience working within a cross functional environment.
• Knowledge of LEAN (or other organizational methodologies) to improve process efficiency and quality a plus.

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About RCM:
RCM IT & Life Sciences is a leading provider of IT Business Solutions and Engineering Services to over 1,000 clients in the commercial and government sectors. RCM partners with clients to define, implement and manage a broad range of technologies across multiple platforms, systems, and networks. Our broad geographic presence ensures that a proven and reliable tactical and strategic capability is available and deployable virtually everywhere in North America.
Equal Opportunity Statement:
RCM is an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.
Disclaimer:
This job description is intended to describe the general nature and the level of the work being performed by the people assigned to this position. It is not intended to include every job, duty, and responsibility specific to the position. RCM reserves the right to amend and change responsibilities to meet business and organizational needs, as necessary.