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Form of workMedical Technologist at the MGH Cancer Center in the Immune Monitoring Laboratory. We are looking for a highly motivated individual to support the development of this service-orientated, clinically focused research laboratory. The mission of the laboratory is to provide comprehensive high quality immune monitoring that is innovative and enables translational research of immune-based therapies. We work closely with the Maus Lab, and therefore have a unique opportunity to work on groundbreaking CAR-T clinical trials in additional to studies using FDA approved products. Current trials include the study of the immune response in patients following infusion with CAR-T for treatment of glioblastoma, multiple myeloma and lymphoma.
Principal Duties and Responsibilities
o Process and bank blood, bone marrow, CSF and solid tissues from patients undergoing immune-based therapy.
o Perform multi-color flow cytometry using a 12 color BD FACS Lyric and the spectral Aurora. Perform flow data analysis to monitor CAR T expansion and function.
o Use Dynex DS2 for ELISA automation in addition to quantification of cytokine, soluble receptors and antibodies in serum, plasma and CSF using Luminex and ELLA multiplex platforms.
o Use QIAcube to perform extraction of DNA and RNA from patient samples.
o Use QIAgility and Bio-Rad automation to perform ddPCR for monitoring CAR-T expansion.
o Perform cell sorting using the SONY MA900.
o Support the introduction of new equipment into the laboratory workflow, including validation and verification.
o Train new lab members in the various techniques.
o Perform preventative maintenance and calibration of specialized equipment.
o Contribute to assay development, quality control processes, and technical validation.
o Maintain accurate sample storage and record keeping.
o Contribute to writing method validations, SOPs following document control procedures.
o Contribute to incident investigations.
o Attend and contribute to weekly lab meetings.
o Attend meetings with trial sponsors and clinical teams and contribute to data presentations
B.S. Degree is required. Minimum 2 years of laboratory experience is required and ASCP certification is preferred. Experience working in a CLIA/CAP regulated laboratory would be desirable.
o Experience working with human samples.
o Meticulous and methodical record keeping.
o Ability to pick up techniques quickly.
o Experience working with LIMS systems.
o Experience in assay development and validation.
o Experience with either flow cytometry or molecular diagnostics would be advantageous.
o Knowledge of hematological malignancies would be an advantage.
o Excellent written communication skills and competency in writing SOPs.
o Must be organized and capable of following protocols.
o Good hand and eye coordination and manual dexterity.
o Ability to work well with others.
o Must have demonstrable experience in method development and/or trouble shooting.
o Must be proficient with MS Office programs such as MS Word, PowerPoint, Excel and statistical packages.
o Ability to work independently on designated projects
