- Follow the laboratory's procedures for specimen handling and processing, test analyses, reporting and maintaining records of patient test results;
- Maintain records that demonstrate that proficiency testing samples are tested in the same manner as patient specimens;
- Adhere to the laboratory's quality control policies, document all quality control activities, instrument and procedural calibrations and maintenance performed;
- Follow the laboratory's established policies and procedures whenever test systems are not within the laboratory's established acceptable levels of performance;
- Be capable of identifying problems that may adversely affect test performance or reporting of test results and either must correct the problems or immediately notify the general supervisor, technical supervisor, clinical consultant, or director;
- Document all corrective actions taken when test systems deviate from the laboratory's established performance specifications; and
- If qualified under 493.1489(b)(5), perform high complexity testing only under the onsite, direct supervision of a general supervisor qualified under 493.1461.
b. Living Donor Transplantation (donation of kidney from related or unrelated individual):
i. Isolates and separates lymphocytes from peripheral blood into two major subpopulations (B and T lymphocytes) for determination of HLA antigens via complement dependent cytotoxicity and/or extracts DNA from blood for HLA typing via molecular methodology. ii. Performs crossmatches between recipients and potential living donors using T lymphocytes and/or B lymphocytes. c. Bone Marrow Transplantation (bone marrow donation from related or unrelated individual) i. Extracts DNA from cells or whole blood ii. DNA amplification using designated programs in thermal cycler iii. Performs tests on SSO beads and/or SSP kits and/or real time kits iv. Prepares agarose gels and load DNA into lanes v. Interprets results based on probe and/or band patterns. 2. Performs appropriate absorptions with platelets and autologous cells to further characterize antibody present in patient sera. 3. Records and reviews test results for accuracy and prepares test reports. Completes administrative tasks required for test completion. Notifies appropriate physician and/or NEOB staff (in case of deceased donor organ distribution) and gives reports to Technical Director and Medical Director for verification. 4. Prepares and determines suitability of solutions, reagents, and stains following standard laboratory formulas and procedures. Records control values of technical equipment daily to monitor and ensure their reliability. 5. Participates in competency and monthly proficiency testing. 6. Performs sophisticated calculations in interpretation of certain test results (i.e., p values to determine statistical significance of some tests, correlation coefficients to evaluate the quality of reagent sera, etc.) 7. Utilizes laboratory computer for updating of recipient status on national organ sharing (UNOS) list, for sample entry and test orders, for daily test analysis, and for record-keeping concerning patients and sophisticated reagents. 8. Participates in research, development, and implementation of improved or new Clinical Laboratory techniques and procedures as required. 9. Performs additional molecular testing for determination of specific antigens, such as HLA-B27 and HLA-B*57:01, as diagnostic aids. 10. Teaches laboratory procedures to rotating trainees from other laboratories, pathology residents/fellows, NEOB personnel, and renal immunology fellows on a regular basis.. 11. Keeps current regarding developments in techniques and trends in histocompatibility and immunologic testing through appropriate technical journals, as well as internal and external educational programs. 12. Is responsible for awareness of National Patient Safety Goals and to comply with BWH Hospital and Laboratory policies and procedures. 13. Maintains an orderly work area. 14. Maintains a working familiarity with Sunquest and Epic information systems 15. Performs other duties as required QUALIFICATIONS: 1. Must possess a Bachelor's degree in a chemical, physical, or biological science from an accredited college or university. (There are no organized academic programs which adequately train individuals for work in this field; a broad scholastic background in biology, chemistry, and immunology is preferred.) 2. Must have satisfactorily completed in-house laboratory training/orientation program, including on-call duties. Training requires between nine and twelve months, on average, to acquire the necessary familiarity with laboratory policies, as well as theoretical and practical knowledge of procedures and tests. There are currently no licensure requirements in histocompatibility. s than 100 pounds).