Serve as an Undergraduate Research assistant on the project: Selected Enzymes May Reveal Cardiopathology: LDH, MDH, AChE
The research assistant will learn to prepare samples of normal and heart failure muscle from canis tissues. Thereafter, a standard curve will be developed to normalize loading volumes; specific analysis of 3 key isoenzymes (LDH, MDH, EST); triplicate repeats of protocol.
The research assistant:
-will learn steps in the preparation of multiple samples
-will learn to develop a standard curve on the computer for concentration determination and analysis
-is required to master isozyme chemistry basics and protocol for identification of selected specific enzymes; and,
-must use writing skills for detailed date entry and the development of an outline to enable construction of a scientific research paper.