Address
Form of workGENERAL SUMMARY/OVERVIEW STATEMENT:
A position is open for a full-time research assistant (RA) in the Department of Pathology at Brigham and Women's Hospital. Under the supervision of Dr. David Walt and Dr. Travis Gibson, the selected candidate will provide technical assistance for research pertaining to the human gut microbiome and its interactions with the host immune system. This position will provide the opportunity to work with histologic, immunohistochemical, molecular cytogenetic, and in situ protocols in a state-of-the-art lab environment.
Among other responsibilities, the selected candidate will perform multiplexed error-robust fluorescence in situ hybridization (MERFISH). The RA will perform all stages of the MERFISH protocol, including tissue fixation, permeabilization, hybridization, embedding, and clearing, in addition to imaging using a custom-built semi-automated robotic system built by a team at BWH. The selected candidate will primarily work independently to prepare and process tissue samples while optimizing the respective protocols, and will work closely with the surrounding research team to interpret the resulting data. Previous experience with protocols involving RNA is considered a strong asset. Other responsibilities of the selected candidate include assistance with the execution of the ultrasensitive single molecule array (Simoa) assay. Research conducted by the candidate in collaboration with the research team will provide new insights into the dynamics of the gut microbiome and will address underlying biological questions.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
The responsibilities of the selected candidate may include:
1. Independently performing routine and non-routine experimental protocols of moderate to high complexity. Experimental work will include preparing buffers and reagents without RNase contamination and evaluating the reagents for viability.
2. Performing literature searches relevant to the execution of the required protocols.
3. In collaboration with the PIs or Research Manager, modifying existing research techniques and potentially establishing new techniques.
4. Communicating progress professionally with collaborators and with the scientific community.
5. Coordinating and scheduling tests and procedures, and documenting experimental work accurately and in detail.
6. Ordering laboratory supplies related to their assigned tasks.
7. Following all lab safety protocols.
QUALIFICATIONS:
BA/BS in biological/physical science required. Some prior research preferred but not essential.
SKILLS/ ABILITIES/COMPETENCIES REQUIRED:
- Sound analytical and organizational skills.
- Requires good oral and written communication skills.
- Must be able to logically and effectively structure tasks and set priorities.
- Ability to identify potential problems and troubleshoot solutions.
WORKING CONDITIONS:
Normal laboratory environment. Lab contains postdoctoral fellows, residents, graduate students, technicians, and lab aides. Minimal risk when established protocols are followed.
