[Based in China] CAR-T Technology Platforms Scientist (Multiple Levels)

Legend Biotech is seeking CAR-T Technology Platforms Scientist (Multiple Levels) as part of the R&D teamBased inNanjing, China. 

Key Responsibilities  

• Develop and optimize cell therapy platforms, e.g., CAR-NK, CAR-T, to improve the biological function (potency and persistence) of cell therapy products, based on solid understanding of innate cell biology, i.e. immune cell activation, survival, proliferation, and function, and cancer/immune biology in tumor microenvironment.
• Lead from the lab to advance novel and differentiated cell therapy products toward clinical milestones, by working collaboratively with internal cross-functional teams and external partners.
• Contribute to discovery projects by independently designing and developing well-defined mechanistic experiments and projects.
• Design, execute, and interpret functional in vivo, in vitro, and ex vivo assays to understand the mechanism-of-action and play an active role in subsequent scientific discussions to drive decisions.
• Independently design sgRNAs, constructs for KO and KI through non-viral (plasmid, self-replicating vectors, EP, LNP) methods.
• Develop characterization strategies and implement plans to understand the cellular biology with respect to growth/expansion of cellular subtypes during process/product development in both autologous and allogenic production processes.
• Propose novel projects based on internal results, external partners, and published reports and demonstrate leadership through effective communication of study plans/value, and research results.

• Ph.D. in Immunology, Biology, Biological/Chemical Engineering, or related discipline with 2+ years of relevant industry/academic experience post-graduation
• Hands on experience with CAR-T, CAR-NK, CAR-T, TCR-T cell therapies / immunotherapies, including cell culture, immune cell activation/expansion, viral transduction for various types of immune cells.
• Profound skills in in vitro and in vivo immune cell characterization and function assessment, such as, multi-color flow immunophenotype, multiplex cytokine measurement, cytotoxicity assay, and other molecular based assays.
• Experience with in vitro technologies to assess lymphocyte functionality including but not limited to multi-parameter flow cytometry, MSD, Luminiex, xCELLigence and IncuCyte-based assays, for phenotypic characterization of T/NK cells, T/NK cell activation, cytotoxicity and proliferation assays is required.
• Experience with gene editing in primary cells is preferred (eg. CRISPR-CAS9 editing, lentiviral transduction, retrovirus transduction)
• Excellent written and verbal communication skills, detail-oriented organization skills, and team collaboration skills.

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