Job Description
Role Summary
We are seeking a Scientist I, Cancer Biology and Translational Sciences with a strong scientific expertise in T/NK cell biology and/or Cellular Therapy and some knowledge in the mechanisms that regulate T cell- and NK cell-mediated alloreactivity The. candidate will be part of a highly cross functional Cancer Biology and Translational Sciences group in Seattle. The Cancer Biology group works closely with multiple groups including Protein and Cell Engineering, Stem Cell and Developmental Immunology, Process Development and Computational Biology groups to develop in vitro models for assessing the function of engineered T-cells and understanding the clearance of allogeneic T-cell products. Successful candidate will develop and execute scientific strategies for the development of allogeneic cell therapies. Scientist in this role would focus on developing strategies to overcome the immune response that can occur with iPSC derived cell therapies.
About Notch (www.notchtx.com)
Notch is an early-stage biotech company, working to maximize the benefit of cell therapies through a proprietary T cell-production platform which combines sophisticated product design with commercial-compatible processes to enhance patient outcomes. Notch’s iPSC-based technology platform allows for precision control of notch signaling, which removes several critical limiting factors in the development of cell therapies, delivering the ability to design and manufacture a uniform and unlimited supply of therapeutic T cells.
Responsibilities:
- Identify and bank PBMCs with various degrees of mismatch to Notch’s iPSC lines.
- Expertise in processing and banking cells from Leukopaks.
- Perform CRISPR based gene manipulations in primary T cells.
- Design and execute experiments to evaluate the efficacy of allogeneic iPSC derived immune cells in MLR assays.
- Execute experiments to test novel approaches to assess allogeneic clearance of iPSC derived cells by T/NK cells.
- Provide strategic input and scientific direction in the development of novel allogeneic cell therapies.
- Lead, design, coordinate and execute proof-of-concept research with timely data analysis, interpretation, and communication to key stakeholders.
- Perform in vitro functional assays (including cytokine release/degranulation and Incucyte based short/long-term killing assays)
- Work with colleagues to maintain laboratory in good working order as well as uphold standards of safety.
- Maintain electronic laboratory notebooks and other documentation as need for regulatory filings.
Required Qualifications and Experience:
- PhD with 2+ years of experience.
- Background in immunology.
- Proficient in culturing and manipulating human immune primary cells and cell lines
- Experience working with engineered T-cells (CAR/TCR).
- Experience with Electronic Laboratory Notebooks (ELN)
- Practical experience with gene-editing and/or gene-engineering concepts is preferred, but not required.
- Hands-on expertise in NK/T-cell functional characterization assays as in vitro cytotoxicity, proliferation, cytokine measurement.
- Expertise design and performing functional assays, cell transfection/ transduction in immune cells.
- Ease with multi-parameter flow cytometry (Panel Development and Data Analysis)
- Critical thinking and an ability to read and analyze scientific publications in the field.
- Experience handling multiple priorities with a focus on alignment with company goals.
- Experience in working with allogeneic systems and familiar with assays as Mixed Lymphocyte Reaction (MLR), and NK and/or T cells cytotoxicity assays.
- Willingness to think outside the box, be creative and ability to work in a very fast paced, startup environment.
Salary Range: $106,000 - $137,000
Benefits:
- Paid Vacation and Sick time
- Hybrid Work Framework
- Healthcare plans
- 401k plan
- Commuting Financial Assistance
- Home Office WIFI Support
- Employee assistance programs (EAPs)
- Internal learning & development opportunities
Notch Therapeutics Inc. is an equal opportunity employer. All qualified applications will receive consideration for employment without regard to race, age, gender identity, sexual orientation, color, religion, sex, marital status, national origin, protected veteran status, disability status, or any other status protected by federal, provincial or local law.
Requests for accommodation can be made at any stage of the recruitment process providing the applicant has met the Bona-fide requirements for the open position. Applicants need to make their requirements known when contacted.